Zoology and Animal physiology (ZFZd)
Activation of endogenous mechanisms leading to neuroprotection after stroke
Stroke represents a serious socio-economic problem with limited treatment options. Recently, the phenomenon of ischemic tolerance, i.e. endogenous stimulation of the mechanisms with the ability to induce neuroprotection, has become an attractive solution for the prevention and treatment of such conditions.
1) Study of mechanisms of ischemic tolerance 2) Defining the role of peripheral blood cells in inducing ischemic tolerance 3) Testing of in vivo and ex vivo conditioning methods 4) Testing of conditioning methods in animal models of ischemic-reperfusion injury of nerve tissue
Bonova, P., Jachova, J., Nemethova, M., Macakova, L., Bona, M., Gottlieb, M., 2020. Rapid remote conditioning mediates modulation of blood cell paracrine activity and leads to the production of a secretome with neuroprotective features. Journal of neurochemistry 154, 99-111. (2) Bonova, P., Koncekova, J., Nemethova, M., Petrova, K., Bona, M., Gottlieb, M., 2022. Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats. Biomolecules 12. (3) Bonova, P., Nemethova, M., Matiasova, M., Bona, M., Gottlieb, M., 2016. Blood cells serve as a source of factor-inducing rapid ischemic tolerance in brain. The European journal of neuroscience 44, 2958-2965. (4) Hossmann, K.A., 2012. The two pathophysiologies of focal brain ischemia: implications for translational stroke research. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 32, 1310-1316.
RNDr. Petra Bonová, PhD.
Molecular Cytology and Genetics (MCGd)
OSMAC (One Strain Many Compounds) approach assisted activation of fungal endophytes for the production of anticancer drugs
The OSMAC (One Strain Many Compounds) approach represents a significant strategy in drug discovery, particularly in leveraging the potential of fungal endophytes to generate anticancer compounds. Fungal endophytes are a valuable source of bioactive compounds, including various agents known for their anticancer properties. Central to the OSMAC approach is the principle of enhancing metabolite diversity. This enhancement is achieved through various techniques, such as media variation and environmental manipulations, distinguishing it from traditional methods of metabolite production. This doctoral thesis will investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in selected Hypericum-associated endophytic fungi, utilizing the OSMAC approach. The research aims to optimize the OSMAC approach by adjusting various physicochemical culture parameters and incorporating nature-mimetic abiotic and ecological niche-mimetic biotic stress factors. Key physicochemical parameters include temperature, pH, nitrogen source, carbon source, light, and aeration. The OSMAC experiments will integrate feeding studies using unlabeled and labeled methodologies, along with triggers and inducers to activate BGCs, guided by sequencing and annotation data. Expression profiling and compound-structure-informed transcriptomics will be conducted on enhanced endophytes, combining these with in silico predictions and molecular docking analyses of target anticancer compounds. A bioassay-guided analysis will examine fungal extracts and fractions in collaboration with partner labs, using advanced tools like high-resolution mass spectrometry and NMR. This process aims to create a chemical fingerprint of bioactive compounds from endophytic fungi, cataloging key metabolites and target anticancer drugs, such as anthraquinones. Bioassay-guided metabolomics will also explore interactions between Hypericum phytopathogens and endophytes, identifying synergistic or antagonistic compounds. The OSMAC approach will help exclude inactive compounds while focusing on promising anticancer metabolites. This doctoral thesis aims to utilize OSMAC-enhanced fungal endophytes to produce clinically relevant anticancer drugs, particularly anthraquinones sustainably.
This doctoral thesis will investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in selected Hypericum-associated endophytic fungi, utilizing the OSMAC approach. Overall, this doctoral thesis aims to utilize OSMAC-enhanced fungal endophytes to produce clinically relevant anticancer drugs, particularly anthraquinones sustainably.
See publications of the PI: https://www.upjs.sk/en/PF/employee/souvik.kusari/
Priv.-Doz. Souvik Kusari, Dr. rer. nat., univerzitný profesor
prof. RNDr. Eva Čellárová, DrSc.
Molecular Cytology and Genetics (MCGd); Molecular Cytology and Genetics (MCGd)
Assessment of the employment of paraarterial spaces of glymphatic system as the alternative administration route for pharmacotherapy of CNS
The glymphatic system is a recently discovered system represented by paravascular spaces in the central nervous system (CNS), which promotes efficient elimination of soluble proteins and metabolites from the nervous tissue to the cerebrospinal fluid (CSF). Understanding of organization and physiology of the glymphatic system could have an impact on the development in the field of pharmacotherapy of the CNS pathologies. PhD study should focus on the study of the glymphatic system and its utilization for the administration of the therapeutics in the treatment of CNS diseases. The first aim is to analyze the components of the glymphatic system during the ontogenesis, focusing on characterization of attributes, which are important for maintenance of homeostasis and for its potential utilization in the pharmacotherapy. The second aim is to investigate, whether the glymphatic system could be utilized for the intracisternal application of therapeutics loaded into the biodegradable nanoparticles based on silicon dioxide, which could overcome restrictions related to the application of drugs via hematoencephalic barrier, or conventional administration of drugs via intracisternal or intrathecal route.
1. Analysis of components of glymphatic system in the telencephalon and spinal cord: paravascular space (Virchow-Robin paraarterial space, paravenous space), cells (astrocytes, microglia, ependymal cells) and CSF during ontogenesis of rats 2. Analysis of the perspective utilization of intracisternal application of therapeutics loaded in silica based biodegradable nanoparticles in the treatment of CNS diseases 3. Study of the impact of administration of biodegradable silica based nanoparticles on the functions of the nervous and glymphatic systems
scientific studies published in scholar journals
doc. RNDr. Juraj Ševc, PhD.
RNDr. Anna Alexovič Matiašová, PhD.
Molecular Cytology and Genetics (MCGdAj); Molecular Cytology and Genetics (MCGdAj)
Assessment of the employment of paraarterial spaces of glymphatic system as the alternative administration route for pharmacotherapy of CNS
The glymphatic system is a recently discovered system represented by paravascular spaces in the central nervous system (CNS), which promotes efficient elimination of soluble proteins and metabolites from the nervous tissue to the cerebrospinal fluid (CSF). Understanding of organization and physiology of the glymphatic system could have an impact on the development in the field of pharmacotherapy of the CNS pathologies. PhD study should focus on the study of the glymphatic system and its utilization for the administration of the therapeutics in the treatment of CNS diseases. The first aim is to analyze the components of the glymphatic system during the ontogenesis, focusing on characterization of attributes, which are important for maintenance of homeostasis and for its potential utilization in the pharmacotherapy. The second aim is to investigate, whether the glymphatic system could be utilized for the intracisternal application of therapeutics loaded into the biodegradable nanoparticles based on silicon dioxide, which could overcome restrictions related to the application of drugs via hematoencephalic barrier, or conventional administration of drugs via intracisternal or intrathecal route.
1. Analysis of components of glymphatic system in the telencephalon and spinal cord: paravascular space (Virchow-Robin paraarterial space, paravenous space), cells (astrocytes, microglia, ependymal cells) and CSF during ontogenesis of rats 2. Analysis of the perspective utilization of intracisternal application of therapeutics loaded in silica based biodegradable nanoparticles in the treatment of CNS diseases 3. Study of the impact of administration of biodegradable silica based nanoparticles on the functions of the nervous and glymphatic systems
scientific studies published in scholar journals
doc. RNDr. Juraj Ševc, PhD.
RNDr. Anna Alexovič Matiašová, PhD.
Molecular Cytology and Genetics (MCGdAj)
Biodiversity and bioprospecting of halophilic archaea from miocene salt deposits
Bioprospecting of extreme environments provides clear evidence that extreme conditions naturally select microorganisms with modified biochemistry giving opportunity for new compounds, genes, and processes discoveries. The aim of the project is an in-depth exploration of the uncharted realm of halophilic archaea, focusing on the Miocene salt deposits as a unique and understudied environment. The Miocene salt deposits could serve as a natural repository of ancient genetic material, providing insights into the evolutionary history and ecological roles of halophilic archaea. By combination of advanced molecular techniques and culturing methods, an array of halophilic archaea from these deposits will be isolated and characterized. The bioprospecting potential of these halophilic archaea with applications in modern biotechnology, pharmaceuticals, and sustainable industries will be analyzed. Novel enzymes, biosurfactants, and bioactive compounds will be identified in addressing pressing environmental challenges. The study will contribute significantly to the expanding knowledge of halophilic archaea ecosystems and highlights the protection and sustainable utilization of these microbial resources for future biotechnological advancements.
The aim of thesis is using cultivation approaches analyze the archaea populations from miocene salt deposits
McGenity, T.J.; Oren, A. Hypersaline Environments. In Life at Extremes: Environments, Organisms and Strategies for Survival; Bell, E.M., Ed.; CAB International: Oxfordshire, UK, 2012 Oren, A. Life in Hypersaline Environments. In Their World: A Diversity of Microbial Environments, Advances in Environmental Microbiology; Hurst, C.J., Ed.; Springer International Publishing: Cham, Switzerland, 2016 Ventosa, A.; Márquez, M.C.; Sánchez-Porro, C.; de la Haba, R.R. Taxonomy of Halophilic Archaea and Bacteria. In Advances in Understanding the Biology of Halophilic Microorganisms; Vreeland, R.H., Ed.; Springer: Dordrecht, The Netherlands, 2012 Current scientific literature
doc. RNDr. Peter Pristaš, CSc., univerzitný profesor
RNDr. Jana Kisková, PhD.
Molecular Cytology and Genetics (MCGd)
Biodiversity and bioprospecting of halophilic archaea from miocene salt deposits
Bioprospecting of extreme environments provides clear evidence that extreme conditions naturally select microorganisms with modified biochemistry giving opportunity for new compounds, genes, and processes discoveries. The aim of the project is an in-depth exploration of the uncharted realm of halophilic archaea, focusing on the Miocene salt deposits as a unique and understudied environment. The Miocene salt deposits could serve as a natural repository of ancient genetic material, providing insights into the evolutionary history and ecological roles of halophilic archaea. By combination of advanced molecular techniques and culturing methods, an array of halophilic archaea from these deposits will be isolated and characterized. The bioprospecting potential of these halophilic archaea with applications in modern biotechnology, pharmaceuticals, and sustainable industries will be analyzed. Novel enzymes, biosurfactants, and bioactive compounds will be identified in addressing pressing environmental challenges. The study will contribute significantly to the expanding knowledge of halophilic archaea ecosystems and highlights the protection and sustainable utilization of these microbial resources for future biotechnological advancements.
The aim of thesis is using cultivation approaches analyze the archaea populations from miocene salt deposits
McGenity, T.J.; Oren, A. Hypersaline Environments. In Life at Extremes: Environments, Organisms and Strategies for Survival; Bell, E.M., Ed.; CAB International: Oxfordshire, UK, 2012 Oren, A. Life in Hypersaline Environments. In Their World: A Diversity of Microbial Environments, Advances in Environmental Microbiology; Hurst, C.J., Ed.; Springer International Publishing: Cham, Switzerland, 2016 Ventosa, A.; Márquez, M.C.; Sánchez-Porro, C.; de la Haba, R.R. Taxonomy of Halophilic Archaea and Bacteria. In Advances in Understanding the Biology of Halophilic Microorganisms; Vreeland, R.H., Ed.; Springer: Dordrecht, The Netherlands, 2012 Current scientific literature
doc. RNDr. Peter Pristaš, CSc., univerzitný profesor
RNDr. Jana Kisková, PhD.
Zoology and Animal physiology (ZFZd)
The Gut Microbiome as a Therapeutic Target: Multi-Omics Insights into Its Role in Intestinal and Systemic Disorders
In recent years, research on the human microbiome has undergone a revolution, with increasing evidence suggesting that our health is not solely determined by genetics but also by the composition of microorganisms that coexist with us. The gut microbiome has attracted the most attention, as it plays a crucial role in the development and proper functioning of the immune system.When the delicate balance of gut bacteria is disrupted—a condition known as dysbiosis—it can contribute to the onset of severe diseases, including inflammatory bowel diseases (e.g., ulcerative colitis), metabolic disorders, and even neurodevelopmental conditions. One of the most fascinating discoveries of the past decade is the gut-brain axis, a bidirectional communication pathway between the gut microbiota and the brain. This connection is believed to influence not only central nervous system (CNS) development but also behavior and the pathogenesis of autism spectrum disorders (ASD). Given these insights, microbial-based therapeutic approaches, such as fecal microbiota transplantation (FMT), probiotics, and synbiotics, are gaining increasing attention as potential novel treatment strategies for these complex diseases. However, their full potential remains largely unexplored. If you are eager to be part of a pioneering research project that bridges immunology, neuroscience, and microbiology, this PhD opportunity is for you. Our study aims to unravel the mechanisms underlying these disorders by utilizing state-of-the-art molecular biology techniques, including next-generation sequencing (NGS), RNA sequencing (RNA-seq), and other advanced high-throughput analytical methods.Be part of groundbreaking research that has the potential to develop novel therapeutic interventions and significantly impact the future of medicine. This PhD topic is perfect for ambitious students who are driven to push the boundaries of scientific discovery and seek answers to questions that could transform lives.
The main objective of this study is to investigate the impact of the gut microbiome and metabolic processes on the progression and treatment of ulcerative colitis and autism spectrum disorders (ASD). To achieve this, an innovative gnotobiotic animal model will be employed, allowing for a detailed analysis of the mechanisms influencing the development and progression of these conditions.
Specialized literature and scientific publications on the given topic.
RNDr. Vlasta Demečková, PhD., univerzitná docentka
Zoology and Animal physiology (ZFZd)
Diversity and ecology of springtails (Hexapoda, Collembola) in the cave entrances at larger geographic scale
1.) To evaluate the diversity and structure of Collembola communities along a pronounced microclimatic gradients at the cave entrances in the Western Carpathians and Balkan Peninsula. 2.) To analyse distributional patterns of Collembola functional groups at topographically and microclimatically contrasting sites across the cave entrances.
RNDr. Natália Raschmanová, PhD., univerzitná docentka
prof. RNDr. Ľubomír Kováč, CSc.
Molecular Cytology and Genetics (MCGdAj)
Ecogenetics and ecogenomics of extreme environments
Ecogenetics and ecogenomics are emerging fields that explore the intricate relationships between genetic variation and environmental factors, particularly in extreme environments such as deep-sea vents, polar regions, and salt pans and springs. These extreme conditions exert unique selective pressures that shape the genetic architecture of organisms, driving adaptations at both the phenotypic and molecular levels. The aim of the project is to uncover the mechanisms of adaptation, resilience, and diversity among procaryotic hypersaline extremophiles by integrating ecological and genomic data. The data obtained will not only enhance our understanding of microorganisms biodiversity and evolutionary processes but also has should have implications for biotechnological applications, environmental conservation, and understanding potential life forms beyond Earth.
The aim of the thesis is to study the genetic mechanisms of adaptation to extreme environments in prokaryotic organisms using a combination of cultivation and non-cultivation approaches.
Hurst, C.J., Ed.; Their World: A Diversity of Microbial Environments, Advances in Environmental Microbiology; Springer International Publishing: Cham, Switzerland, 2016 Graham, A. and Kopania, E. Genomics of Adaptations to Extreme Environments.Genome Biology and Evolution, 16, 2024: Current scientific literature
doc. RNDr. Peter Pristaš, CSc., univerzitný profesor
RNDr. Jana Kisková, PhD.
Molecular Cytology and Genetics (MCGd)
Ecogenetics and ecogenomics of extreme environments
Ecogenetics and ecogenomics are emerging fields that explore the intricate relationships between genetic variation and environmental factors, particularly in extreme environments such as deep-sea vents, polar regions, and salt pans and springs. These extreme conditions exert unique selective pressures that shape the genetic architecture of organisms, driving adaptations at both the phenotypic and molecular levels. The aim of the project is to uncover the mechanisms of adaptation, resilience, and diversity among procaryotic hypersaline extremophiles by integrating ecological and genomic data. The data obtained will not only enhance our understanding of microorganisms biodiversity and evolutionary processes but also has should have implications for biotechnological applications, environmental conservation, and understanding potential life forms beyond Earth.
The aim of the thesis is to study the genetic mechanisms of adaptation to extreme environments in prokaryotic organisms using a combination of cultivation and non-cultivation approaches.
Hurst, C.J., Ed.; Their World: A Diversity of Microbial Environments, Advances in Environmental Microbiology; Springer International Publishing: Cham, Switzerland, 2016 Graham, A. and Kopania, E. Genomics of Adaptations to Extreme Environments.Genome Biology and Evolution, 16, 2024: Current scientific literature
doc. RNDr. Peter Pristaš, CSc., univerzitný profesor
RNDr. Jana Kisková, PhD.
Molecular Cytology and Genetics (MCGdAj)
Epigenetic modulation of Hypericum spp.-associated endophytic fungi for biotechnological production of anticancer compounds
Hypericin is a photodynamic compound recognized for its pharmacological properties, which include antineoplastic, antiviral, and antibacterial activities. In plant kingdom, it is exclusively found in some species of the genus Hypericum but it is frequently present, along with related anthraquinones in fungal endophytic microorganisms. The complete biosynthetic pathway of hypericin and the genes coding for key enzymes remains largely unknown. Additionally, the genetic and epigenetic regulation of the precursors, reactive intermediates, and the produced anthraquinone have not been extensively studied in plants or fungi. This doctoral thesis aims to investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in specific Hypericum-associated endophytic fungi through in vitro epigenetic modulation, focusing on anticancer anthraquinones. The research will primarily utilize two endophytic fungal strains, Canariomyces subthermophilus and Diaporthe eres, previously identified as producers of hypericin and related anthraquinones. Small-molecule chemical epigenetic modulators will be systematically employed to enhance the endophytic fungal epigenome, promoting targeted chromatin remodeling to activate BGCs that produce anticancer anthraquinones and other drugs. Epigenetic stimulation will be combined with feeding experiments that utilize both unlabeled and labeled approaches alongside triggers and inducers for the activation of BGCs informed by sequencing and annotation data. Additionally, expression analysis and compound-structure-informed transcriptomics of the enhanced endophytes will be conducted, integrating these methods with in silico computational predictions of target anticancer compounds and molecular docking studies. The overarching objective is to utilize epigenetically enhanced fungal endophytes as sustainable sources for producing clinically relevant anticancer drugs, particularly anthraquinones.
This doctoral thesis aims to investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in specific Hypericum-associated endophytic fungi through in vitro epigenetic modulation, focusing on anticancer anthraquinones. The overarching objective is to utilize epigenetically enhanced fungal endophytes as sustainable sources for producing clinically relevant anticancer drugs, particularly anthraquinones.
See publications of the PI: https://www.upjs.sk/en/PF/employee/souvik.kusari/
Priv.-Doz. Souvik Kusari, Dr. rer. nat., univerzitný profesor
prof. RNDr. Eva Čellárová, DrSc.
Molecular Cytology and Genetics (MCGd)
Epigenetic modulation of Hypericum spp.-associated endophytic fungi for biotechnological production of anticancer compounds
Hypericin is a photodynamic compound recognized for its pharmacological properties, which include antineoplastic, antiviral, and antibacterial activities. In plant kingdom, it is exclusively found in some species of the genus Hypericum but it is frequently present, along with related anthraquinones in fungal endophytic microorganisms. The complete biosynthetic pathway of hypericin and the genes coding for key enzymes remains largely unknown. Additionally, the genetic and epigenetic regulation of the precursors, reactive intermediates, and the produced anthraquinone have not been extensively studied in plants or fungi. This doctoral thesis aims to investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in specific Hypericum-associated endophytic fungi through in vitro epigenetic modulation, focusing on anticancer anthraquinones. The research will primarily utilize two endophytic fungal strains, Canariomyces subthermophilus and Diaporthe eres, previously identified as producers of hypericin and related anthraquinones. Small-molecule chemical epigenetic modulators will be systematically employed to enhance the endophytic fungal epigenome, promoting targeted chromatin remodeling to activate BGCs that produce anticancer anthraquinones and other drugs. Epigenetic stimulation will be combined with feeding experiments that utilize both unlabeled and labeled approaches alongside triggers and inducers for the activation of BGCs informed by sequencing and annotation data. Additionally, expression analysis and compound-structure-informed transcriptomics of the enhanced endophytes will be conducted, integrating these methods with in silico computational predictions of target anticancer compounds and molecular docking studies. The overarching objective is to utilize epigenetically enhanced fungal endophytes as sustainable sources for producing clinically relevant anticancer drugs, particularly anthraquinones.
This doctoral thesis aims to investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in specific Hypericum-associated endophytic fungi through in vitro epigenetic modulation, focusing on anticancer anthraquinones. The overarching objective is to utilize epigenetically enhanced fungal endophytes as sustainable sources for producing clinically relevant anticancer drugs, particularly anthraquinones.
See publications of the PI: https://www.upjs.sk/en/PF/employee/souvik.kusari/
Priv.-Doz. Souvik Kusari, Dr. rer. nat., univerzitný profesor
prof. RNDr. Eva Čellárová, DrSc.
Zoology and Animal physiology (ZFZd)
Experimental strategies for the treatment of paraplegia caused by spinal cord injury
There is currently no effective therapy to reduce neurological dysfunction caused by spinal cord injury (SCI The proposed dissertation will utilize various treatment approaches such as pharmacological interventions and rehabilitation to facilitate recovery of function after SCI. The effect of the treatments on the regeneration of the function will be evaluated by functional, molecular and morphological techniques. The aim of the dissertation will be to investigate the mechanism and impact of post-traumatic treatment. The results of this work may lead to clinical practice in the future.
The aim of the dissertation will be to investigate the mechanism and impact of post-traumatic treatment.
Kucharova K, Stallcup WB. 2017. Distinct NG2 proteoglycan-dependent roles of resident microglia and bone marrow-derived macrophages during myelin damage and repair. PLoS One 12(11): e0187530. https://doi.org/10.1371/journal.pone.0187530 Kucharova K, Stallcup WB. 2015. NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair. J Neuroinflammation 12:161. doi: https://doi.org/10.1186/s12974-015-0385-6 Bradbury EJ and Burnsid ER. 2019. Moving beyond the glial scar for spinal cord repair. Nature communications 10:3879 https://doi.org/10.1038/s41467-019-11707-7 Hu X, Xu W, Ren Y, Wang Z,He X, Huang R, Ma B, Zhao J, Zhu R, Cheng L. 2023. Spinal cord injury: molecular mechanisms and therapeutic interventions. Signal Transduct Target Ther. 26;8(1):245. doi: https://doi.org/10.1038/s41392-023-01477-6 Li Z, Yu S, Hu X, Li Y, You X, Tian D, Cheng L, Zheng M, Jing J (2021) Fibrotic Scar After Spinal Cord Injury: Crosstalk With Other Cells, Cellular Origin, Function, and Mechanism. Front Cell Neurosci. 15:720938. https://doi.org/10.3389/fncel.2021.720938
MUDr. Karolína Kuchárová, PhD.
Zoology and Animal physiology (ZFZd)
Geospatial analysis of occurrence and spread of ixodid ticks in the context of middle Europe
Ticks are among the most dangerous blood-sucking arthropods that parasitize both humans and animals. Their significance is highlighted by the fact that they serve as vectors for several serious diseases. As parasites directly dependent on environmental factors, ticks respond to climate change by altering their distribution and life cycles. This study focuses on one of the most important and rapidly spreading tick species in Central Europe, the ornate cow tick (Dermacentor reticulatus), in Slovakia. A specific phenomenon regarding its distribution is its mosaic-like presence in lowlands and hilly areas, with a strong affinity for riverine environments. The main objective of this project is to conduct spatial analyses and model the relationships between the occurrence and population density of D. reticulatus, vegetation, and environmental factors using geospatial technologies (GIS) with cartographic representation. Special attention will be given to the occurrence of ticks in urban and peri-urban green spaces, as well as their current distribution, spread, and potential expansion across Slovakia.
The primary objectives of this study are: 1. To determine the current distribution of Dermacentor reticulatus in Slovakia in relation to habitat types, vegetation, climate zones, and urban/peri-urban environments. 2. To perform spatial analysis and modeling using geographic information technologies.
Lansdell S., Zorto A., Seto M., Negera E., Sharif S., Cutler S. Insights into the contribution of multiple factors on Ixodes ricinus abundance across Europe spanning 20 years using different machine learning algorithms (2025) Ticks and Tick-borne Diseases, 16 (1), art. no. 102437 Ramsey M.L., Kollath D.R., Antoninka A.J., Barker B.M. Proposed Relationships Between Climate, Biological Soil Crusts, Human Health, and in Arid Ecosystems (2025) GeoHealth, 9 (1), art. no. e2024GH001217 Földvári, G., Široký, P., Szekeres, S. et al. Dermacentor reticulatus: a vector on the rise. Parasites Vectors 9, 314 (2016). https://doi.org/10.1186/s13071-016-1599-x Karbowiak, G, The occurence of the dermacentor reticulatus ticks – its expansion to new areas and possible causes. Annals of Parasitology 214, 60(1), 37-47
RNDr. Igor Majláth, PhD.
doc. Mgr. Michal Gallay, PhD.
Plant physiology (FRd)
Chemical communication between lichens and bryophytes and their response to biotic stress.
Mosses and lichens often share their microhabitats, which suffer from the same environmental changes, i.e. j. both biotic and abiotic interactions. In this issue, we would focus on the relationship between selected species of mosses and lichens, and the goal would be to find out how they communicate chemically. There are many complex interaction types, but our aim would be to explain how mosses respond to the presence of certain lichens, including developmental, biochemical, physiological and chemical changes. The aim of the study will also be the allelopathic effects of lichens on moss spore germination or motility. The expected results are based on the hypothesis that bryophytes are likely to have specific interactions, both positive and negative. In the next step of the study, the overlap of similar interactions will be analyzed to specify the mechanism involved in the pair relationships and the compounds involved in the fine chemical communication between the moss and lichen species tested.
The aim of the dissertation is the study of interactions between lichens and mosses at the level of chemical communication. Another goal of this work is to deepen the knowledge and insights in the field of allelopathy among these strong groups of cryptogams. The study of adaptation and reaction mechanisms at the chemical level will also be the subject of study in this topic.
Scientific articles from databases Web of Science and Scopus
prof. Dr. rer. nat. Marko Sabovljević, Dr. rer. nat.
doc. RNDr. Michal Goga, PhD.
Zoology and Animal physiology (ZFZd)
Tick borne pathogens in urban and natural environmement
Ticks transmit a wide range of pathogens, from viruses and bacteria to parasites, causing diseases in both humans and animals. Their distribution depends on multiple environmental factors, and in recent decades, there has been a significant increase in tick populations, even in urban areas. Urban greening programs include planting trees and flowers, green buildings, and creating parks and forests. These initiatives also involve the establishment of green-blue networks, which connect water bodies with green spaces like agricultural areas, parks, and unused lands. The goal of these measures is to create or preserve biodiversity refuges and facilitate species movement through ecological corridors. However, the relationship between urban greening initiatives and the presence of ticks, which pose potential health risks, is often overlooked. Green spaces in cities form confined microhabitats that provide optimal conditions for animals such as small rodents, birds, and lizards, which play a crucial role as tick hosts in their developmental cycle and act as reservoirs for pathogens. As part of this doctoral research, bacterial pathogens will be monitored, and the involvement of vertebrates in the circulation of these pathogens will be analyzed. The species spectrum of pathogens will be studied using molecular approaches, next-generation sequencing, and subsequent genetic analysis of selected species with the potential to cause diseases. For risk assessment, urban environments will be compared with natural environments, and high-risk zones in cities will be identified.
The main objective of this study is: 1. To determine the quantitative and qualitative characteristics of the spectrum of vectors and pathogens in urban and natural environments. 2. To evaluate the involvement of hosts in pathogen transmission depending on the environment.
Lansdell S., Zorto A., Seto M., Negera E., Sharif S., Cutler S. Insights into the contribution of multiple factors on Ixodes ricinus abundance across Europe spanning 20 years using different machine learning algorithms (2025) Ticks and Tick-borne Diseases, 16 (1), art. no. 102437, Ramsey M.L., Kollath D.R., Antoninka A.J., Barker B.M. Proposed Relationships Between Climate, Biological Soil Crusts, Human Health, and in Arid Ecosystems (2025) GeoHealth, 9 (1), art. no. e2024GH001217 Maldonado-Ruiz P. The Tick Microbiome: The “Other Bacterial Players” in Tick Biocontrol (2024) Microorganisms, 12 (12), art. no. 2451, Rosso F., Ferrari G., Weil T., Tagliapietra V., Marini G., Dagostin F., Arnoldi D., Girardi M., Rizzoli A.Temporal Changes in Tick-Borne Pathogen Prevalence in Questing Ixodes ricinus Across Different Habitats in the North-Eastern Italian Alps (2024) MicrobiologyOpen, 13 (6), art. no. e010 multiple factors on Ixodes ricinus abundance across Europe spanning 20 years using different machine learning algorithms (2025) Ticks and Tick-borne Diseases, 16 (1), art. no. 102437, Ramsey M.L., Kollath D.R., Antoninka A.J., Barker B.M. Proposed Relationships Between Climate, Biological Soil Crusts, Human Health, and in Arid Ecosystems (2025) GeoHealth, 9 (1), art. no. e2024GH001217 Egan S.L., Taylor C.L., Banks P.B., Northover A.S., Ahlstrom L.A., Ryan U.M., Irwin P.J., Oskam C.L. The bacterial biome of ticks and their wildlife hosts at the urban– wildland interface (2021) Microbial Genomics, 7 (12), art. no. 000730.
RNDr. Viktória Majláthová, PhD., univerzitná docentka
Zoology and Animal physiology (ZFZd)
Molecular mechanisms of early embryo cell responses to the factors of environment
The mammalian preimplantation embryo (i.e. the embryo in the period from oocyte fertilization to implantation of blastocyst into uterus) can finish its development to the blastocyst stage relatively autonomously and in in vitro conditions in relatively simple culture media. On the other hand, recent data indicate that disturbed maternal environment significantly affects developmental potential of oocytes, quality of pre-implantation embryos, success of implantation and can be the cause of unsuccessful pregnancies in both humans and animals. Moreover, in line with the DOHaD (Developmental Origin Health and Diseases) hypothesis, impairment of early embryonic development can also have long term consequences for health in adulthood. The research will mainly focus on the role of low molecular weight ligands and their receptors. The experiments will mostly use a mouse model (in vivo as well as in vitro approaches, including mouse embryonic stem cells). Modern biochemical methods, molecular biology techniques as well as morphological methods will be used to analyze cell receptors, activated signaling pathways and physiological responses of early embryo cells.
1) To analyze the expression of selected cell receptors binding low molecular weight ligands in preimplantation embryos 2) To analyze the impact of activation of identified cell receptors on early embryonic cells 3) To analyze the possible long-term effects of activation of identified cell receptors on the physiological / health status of the offspring
DePamphilis M. 2016. Mammalian preimplantation development. Academic Press, Elsevier, 447pp. Leese HJ and Brison DR. 2015 Cell Signaling during mammalian early embryo development. Springer, 213pp. Niakan KK et al. Human pre-implantation embryo development. Development. 2012 Mar;139(5):829-41. doi: 10.1242/dev.060426. Leese HJ .Metabolism of the preimplantation embryo: 40 years on. Reproduction 2012 Apr;143(4):417-27. doi: 10.1530/REP-11-0484. Duranthon V et al. Preimplantation embryo programming: transcription, epigenetics, and culture environment. Reproduction 2008 Feb;135(2):141-50. doi: 10.1530/REP-07-0324.
RNDr. Štefan Čikoš, DrSc.
RNDr. Alexandra Špirková, PhD.
Zoology and Animal physiology (ZFZd)
Molecular-biological analysis of the mechanism of ischemic tolerance in atenuation the consequences of stroke by conditioning
Understanding the mechanisms of neuroprotectivity activation after ischemic brain damage at the molecular level is necessary to understand this process. It is known that peripheral blood reflects changes in gene and protein expression in the brain very sensitively and specifically.
1) To identify potential markers (genes, proteins) of ischemic tolerance activation. 2) To classify and specify genes and proteins in their signalling pathways and creating a reaction map of molecular cascades specific for the mechanism of ischemic tolerance in human blood samples and in a rat model.
Yunoki, Masatoshi, et al. "Ischemic tolerance of the brain and spinal cord: a review." Neurologia medico-chirurgica 57.11 (2017): 590-600. Zhao, Wenbo, et al. "Remote ischemic conditioning: challenges and opportunities." Stroke 54.8 (2023): 2204-2207. Furman, Marek, et al. "Quantitative analysis of selected genetic markers of induced brain stroke ischemic tolerance detected in human blood." Brain Research 1821 (2023): 148590. Bonova, Petra, et al. "Blood cells serve as a source of factor‐inducing rapid ischemic tolerance in brain." European Journal of Neuroscience 44.11 (2016): 2958-2965. Furman, Marek, et al. "Modifications of gene expression detected in peripheral blood after brain ischemia treated with remote postconditioning." Molecular Biology Reports (2022): 1-9.
RNDr. Rastislav Mucha, PhD.
Plant physiology (FRd)
Genome multiplication in plant morphogenesis and associated dynamics of cellular and histological changes.
Genome multiplication, or endopolyploidization, represents the repeated doubling of the entire nuclear genome of a cell or only part of it and is one of the mechanisms of change in the ploidy level at the cellular level. It leads to the formation of polyploid cells and occurs during morphogenesis, i.e. differentiation of various organs of plants. The level of endopolyploidy is the result of the interaction of several factors, such as the systematic position of the plant species, the ploidy level, the genome size and the stage of plant development, the type of tissues and organs, and environmental conditions. Endopolyploidy is phylogenetically determined and is also common in economically important angiosperm families such as Fabaceae, Brassicaceae, Solanaceae or Orchidaceae. Current knowledge about changes in endopolyploidy in plant cells and the associated variability in cell and organ growth is rather fragmentary, obtained mainly by studying the model species Arabidopsis thaliana. The enormous taxonomic and morphological diversity of angiosperms represents an unexplored source of suitable models for the study of morphogenesis associated with endopolyploidy induced changes at the cellular and tissue level. The study of new model plants and objects (cells, tissues and organs) different from A. thaliana will bring new knowledge and a better understanding of the role of endopolyploidy in plant morphogenesis.
The aim of the dissertation will be to characterize the level of endopolyploidy and morphological changes at the level of cells and tissues during morphogenesis of i) stress-induced pathologically altered plant organs, ii) variable morphotypes of normally developing organs in selected model species of selected polysomatic angiosperms. The student will have at his disposal a wide range of research objects, the morphogenesis of which has not yet been studied with regard to the variable level of endopolyploidy. During the study, the doctoral student will master several research methods, e.g. flow cytometry, DIC and fluorescence microscopy, histochemical methods or biological 3D confocal imaging techniques.
Scientific articles from databases Web of Science and Scopus
Mgr. Vladislav Kolarčik, PhD., univerzitný docent
Zoology and Animal physiology (ZFZd)
Amphibians in the anthropogenic landscape
Amphibians, as a threatened group in anthropogenic landscapes, are confronted with numerous ecological factors. These conditions may lead to various adaptive or behavioral changes across populations. Studying these adaptations and changes, with an emphasis on ontogeny and gene flow in selected amphibian populations within anthropogenically modified landscapes, can reveal microevolutionary or phenotypic mechanisms of survival in these conditions.
1) Analyze differences in the ontogenetic parameters of metamorphosis in urban amphibians from different environments under experimental conditions. 2) Analyze the types of barriers in anthropogenic landscapes within populations of selected amphibian species.
Dodd C. K. Jr., 2009: Amphibian ecology and conservation: a handbook of techniques. Oxford University Press, New York. Moreno-Rueda G. & Comas M., 2023: Evolutionary Ecology of Amphibians. CRC Press, Boca Raton. Vitt L. & Caldwell J. P., 2014: Herpetology. An introductory biology of amphibians and reptiles. Fourth edition. Academic Press.
doc. RNDr. Marcel Uhrin, PhD., univerzitný profesor
Mgr. Peter Kaňuch, PhD.
Molecular Cytology and Genetics (MCGdAj)
OSMAC (One Strain Many Compounds) approach assisted activation of fungal endophytes for the production of anticancer drugs
The OSMAC (One Strain Many Compounds) approach represents a significant strategy in drug discovery, particularly in leveraging the potential of fungal endophytes to generate anticancer compounds. Fungal endophytes are a valuable source of bioactive compounds, including various agents known for their anticancer properties. Central to the OSMAC approach is the principle of enhancing metabolite diversity. This enhancement is achieved through various techniques, such as media variation and environmental manipulations, distinguishing it from traditional methods of metabolite production. This doctoral thesis will investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in selected Hypericum-associated endophytic fungi, utilizing the OSMAC approach. The research aims to optimize the OSMAC approach by adjusting various physicochemical culture parameters and incorporating nature-mimetic abiotic and ecological niche-mimetic biotic stress factors. Key physicochemical parameters include temperature, pH, nitrogen source, carbon source, light, and aeration. The OSMAC experiments will integrate feeding studies using unlabeled and labeled methodologies, along with triggers and inducers to activate BGCs, guided by sequencing and annotation data. Expression profiling and compound-structure-informed transcriptomics will be conducted on enhanced endophytes, combining these with in silico predictions and molecular docking analyses of target anticancer compounds. A bioassay-guided analysis will examine fungal extracts and fractions in collaboration with partner labs, using advanced tools like high-resolution mass spectrometry and NMR. This process aims to create a chemical fingerprint of bioactive compounds from endophytic fungi, cataloging key metabolites and target anticancer drugs, such as anthraquinones. Bioassay-guided metabolomics will also explore interactions between Hypericum phytopathogens and endophytes, identifying synergistic or antagonistic compounds. The OSMAC approach will help exclude inactive compounds while focusing on promising anticancer metabolites. This doctoral thesis aims to utilize OSMAC-enhanced fungal endophytes to produce clinically relevant anticancer drugs, particularly anthraquinones sustainably.
This doctoral thesis will investigate the activation of anticancer natural product biosynthetic gene clusters (BGCs) in selected Hypericum-associated endophytic fungi, utilizing the OSMAC approach. Overall, this doctoral thesis aims to utilize OSMAC-enhanced fungal endophytes to produce clinically relevant anticancer drugs, particularly anthraquinones sustainably.
See publications of the PI: https://www.upjs.sk/en/PF/employee/souvik.kusari/
Priv.-Doz. Souvik Kusari, Dr. rer. nat., univerzitný profesor
prof. RNDr. Eva Čellárová, DrSc.
Zoology and Animal physiology (ZFZd)
Regulation of neurogenesis in the adult brain
The largest neurogenic region in the adult brain is the subventricular zone (SVZ) of the lateral ventricles of the brain. Its specific feature is that cells originating in the SVZ migrate long distances along the so-called rostral migration stream to the olfactory bulb, where they are integrated into existing neuronal circuits. Knowledge of the regulatory mechanisms of adult neurogenesis is essential for its potential therapeutic use. The aim of the thesis will be to investigate the mechanisms regulating cell migration in the rat rostral migratory stream based on histological methods and also using in vitro methods.
The aim of the thesis will be to investigate the mechanisms regulating cell migration in the rat rostral migratory stream based on histological methods and also using in vitro methods.
(1) Saghatelyan A. Role of blood vessels in the neuronal migrtion. Semin. Cell Dev. Biol. 2009, 20:744-750. (2) Lim, D.A., Alvarez-Buylla A. The adult ventricular-subventricular zone (V-SVZ) and olfactory bulb (OB neurogenesis. Cold Spring Harb. Perspect Biol. 2016, 8a018820. (3) Garcia-González, D., Khodosevich, K., Watanabe, Y., Rollenhagen, A., Lübke, J.H.R., Monyer H. Serotonergic projections govern postnatal neuroblast migration. Neuron 2017,94:534-549. (4) Martončíková, M., Alexovič Matiašová, A., Ševc, J. Račeková, E. Relationship between blood vessels and migration of neuroblasts in the olfactory neurogenic region of the rodent brain. Int. J. Mol. Sci. 2021, 11506. (5) Angelidis, A., Račeková, E., Arnoul, P., Závodská, M., Raček A., Martončíková, M. Disrupted migration and proliferation of neuroblasts after postnatal administration of angiogenesis inhibitor. Brain Res. 2018, 1698:121-129.
RNDr. Marcela Martončíková, PhD.
Plant physiology (FRd)
Secondary metabolites in the genus Sorbus and ecophysiologically modulated variability in the content of selected phytochemicals.
The genus Sorbus L. s.l. (rowan, family Rosaceae) is a highly valued group of plants with a high content of ethnopharmacologically important natural chemical compounds with positive effects in respiratory and gastrointestinal diseases, rheumatism, as well as cancer and diabetic diseases. The fruits of some species are used as a suitable functional dietary supplement for high amounts of antioxidant substances. The genus Sorbus includes approximately 250 species of trees and shrubs occurring predominantly in the Northern Hemisphere and is known for the occurrence of hybrid polyploid facultatively apomictic species, and by constantly ongoing speciation at the microevolutionary level. Many hybrid types, still without proper description and taxonomic processing, represent a highly valued and poorly explored source of natural phytochemicals with high pharmacological potential.
The aim of the PhD thesis is to characterize secondary metabolites and their variability in the context of species specificity and ecological variation of their localities using a wide range of available methods (separation techniques, HPLC-MS, substance identification, cytogenetics). In particular: 1. To identify and determine the amount of chemical substances in selected organs (leaves, fruits). 2. To clarify the variability in the content of substances depending on the stage of development of organs and depending on ecophysiological conditions. 3. To estimate the potential of microevolutionary processes (hybridization and polyploidization) in an effort to achieve a qualitatively appropriate combination of phytochemicals.
Scientific articles from databases Web of Science and Scopus
Mgr. Vladislav Kolarčik, PhD., univerzitný docent
Plant physiology (FRd)
Stres metabilites in Lotus sp. and their relevance in stress-response.
In stress conditions, production of secondary metabolites ignificantly contribute to stres-defense of plants. Moreover, that compounds have often antibacterial or other biological effects, which can be interesting from a pharmaceutical or medical point of view. The work would be focused on the research of biosynthesis and accumulation of flavonoids and isoflavonoids under stress conditions in the model species Lotus japonicus, in forage-varieties of L. corniculatus, or in other species of this genera. Compounds will be analyzed by chromatographic methods and identified by LC-MS. Their biosynthesis will be evaluated at the level of gene expression of involved biosynthetic enzymes or transcription factors. The Lotus species accumulate the isoflavan vestitol to a considerable extent, and its importance in plants as a defence metabolite. Alternatively, other important isoflavonoids may be also focused.
The aim of the work is the evaluation of the biosynthesis and accumulation of flavonoids and isoflavonoids under stress conditions in the model species Lotus japonicus incl. Its mutant lines. The results will be compared in the forage-varieties of L. corniculatus, or in other species under the different external factors. Substances will be analysed by chromatographic methods and identified by LC-MS. The biosynthesis of these substances will also be monitored at the level of gene expression for biosynthetic enzymes or transcription factors. Tracking the accumulation of vestitol, clarifying its functions in plant defence. Other important isoflavonoids may be also focused.
Scientific articles from databases Web of Science and Scopus
doc. RNDr. Peter Paľove-Balang, PhD.
Plant physiology (FRd)
Study of heavy metal detoxification mechanisms in lichens with a focus on use in soil phytoremediation
Due to the benefits of symbiosis, lichens can grow in habitats where none of the symbionts can survive as a separate organism. Lichens can accumulate heavy metals in amounts that exceed their nutritional requirements, up to 10% of Their dry weight. The project plans to study changes in the composition of low molecular weight compounds, including cysteine, glutathione and phytochelatins, as well as changes in the content of ascorbic acid, which is considered to be one of the most important antioxidants in cells. We will also analyze change in the composition of acids that are part of the Krebs cycle and the production of reactive oxygen and nitrogen species. The expression of selected heavy metal excess responsive proteins (eg heat shock proteins) will be analyzed using Western blotting and RT-PCR techniques.
The main goal of the proposed project is to study the phytoremediation potential of lichens, combined with an understanding of species specific mechanisms of heavy metal toxicity, or tolerance.
Scientific articles from databases Web of Science and Scopus
prof. RNDr. Martin Bačkor, DrSc.
Zoology and Animal physiology (ZFZd)
Thermal tolerance and acclimation of springtails (Collembola, Hexapoda) from contrasting habitats
Regarding temperature tolerance of ectothermic invertebrates, they should be adapted to handle a wider range of conditions in more climatically variable environments. The climatic variability hypothesis (CVH) will be tested on springtails as a model group of arthropods and applied to the habitats of cave entrances. These environments are characterized by temperature and vegetation inversion. According to CVH, we hypothesize that the highest cold and heat resistances (wide temperature ranges) will found in species occupying environments with marked fluctuating temperatures, while the narrow temperature ranges probably will show species, strictly adapted to thermally more stable conditions. Futhermore, the effect of season and acclimation on thermal tolerances of selected relict taxa will be studied. Laboratory acclimation experiments, focused on the study of phenotypic plasticity, could potentially shed more light on the issues of thermal tolerance of tested Collembola species, especially in the context of climate change.
1. To reveal cold and heat tolerance in selected springtails inhabiting climatically different habitats of cave entrances. 2. To assess season and acclimation effect on thermal tolerances (form of phenotypic plasticity) of selected springtails from contrasting habitats.
RNDr. Natália Raschmanová, PhD., univerzitná docentka
prof. RNDr. Ľubomír Kováč, CSc.
Zoology and Animal physiology (ZFZde)
Tribe Leptodirini, Lacordaire, 1854 (Coleoptera, Leiodidae, Cholevinae) from the Europe and Middle East
After the recent phylogenetic classification, tribe Leptodirini (Coleoptera, Leiodidae, Cholevinae) is divided into two subtribes, Adelopsellina Fresneda et al. 2024 and Leptodirina Lacordaire, 1854. Their geographical range covers the Mediterranean area, expanded from the Iberian Peninsula to Iran. An exception is the third subtribe Platycholeina Horn, 1880, representatives of which are distributed in North America, ranging from British Columbia to California. Two subtribes from the Europe and Near East involve hyperdiverse adaptive radiations of Coleoptera with 229 genera and almost 1340 species and subspecies (Fresneda et al. 2024). They are typically found in different subterranean environments as are caves, pits, superficial subterranean habitats, mines and deep soil. These taxa have very reduced eyesight or are completely blind and exhibit other various adaptations to thrive in twilight or dark cave zones with oligotrophic conditions.
1. complex analysis of two subterranean subtribes of Leptodirini - Adelopsellina a Leptodirina: species diversity, biology, evolution and patterns of geographical distribution, 2. population analysis of the highly adapted subterranean Anthroherpon cylindricollis Apfelbeck, 1889: morphology, molecular traits and phylogeny, 3. population analysis of the highly adapted subterranean Speoplanes biocovensis bosnicus Vrbica et al. 2024: morphology, molecular traits and phylogeny, 4. revision of Katobatizon Knirsch, 1928, a genus highly adapted to subterranean environment.
1. Fresneda, J., Giachino, P. M., Salgado, J. M., Faille, A., Bourdeau, C., Cieslak, A. e Ribera†, I. (2024) A phylogenetic classification of Leptodirini (Coleoptera, Leiodidae, Cholevinae), Memorie della Società Entomologica Italiana, 101 (1), 3–936. 2. Gunn, J. (2004) Encyclopedia of caves and karst science. ISBN: 1579583997, Fitzroy Dearborn 3. Hlaváč, P., Perreau, M. & Čeplík, D. (2017) The subterranean beetles of the Balkan peninsula. Czech University of Life Sciences Prague, Faculty of Forestry and Wood Sciences 4. Jeannel, R. (1924) Monographie des Bathysciinae. Archives de Zoologie expérimentale et générale, 63 (1), 436 pp. 5. Moldovan, O.T., Kováč, Ľ. & Halse, S. (2018) Cave Ecology. Ecological studies 235, Springer Nature 6. Perreau, M. (2015) Leiodidae. In: Löbl, I. & Löbl, D. (Eds.), Catalogue of Palaearctic Coleoptera. Hydrophiloidea—Staphylinoidea. Brill
prof. RNDr. Ľubomír Kováč, CSc.
Zoology and Animal physiology (ZFZd)
The effect of polyunsaturated fatty acids on preimplantation embryo development
Large numbers of people are affected by infertility in their lifetime, according to a new report published by WHO. Around 17.5% of the adult population – roughly 1 in 6 worldwide – experience infertility. Furthermore, in recent decades, many studies have reported a worrisome decrease in the reproductive performance of farm animals. Embryo losses in the early stages of development represent a difficult problem for farmers, as the fundamental element of meat and dairy production is the successful reproduction of animals. However, by managing nutrition during the periconceptional period the incidence of losses can be decreased considerably. Dietary lipid supplementation can be a good strategy to reduce the extent of reproductive failure. Research has shown that polyunsaturated fatty acids (PUFAs) are extremely important and almost essential during pregnancy, especially in the last trimester. These acids are crucial for the proper development of the fetus's brain and eyes. Some of them not only pass through the placenta into the body of the growing fetus, but after birth continue into the body of the newborn through breast milk. Their presence is essential for the further development of the individual. The main aim of the study will be to evaluate the effect of diet supplementation with PUFAs, on the ability to conceive and to reduce early embryo loss in animal model - mouse females. The complementary set of relevant and up-to-date physiological, morphological, biochemical and molecular biology methods will be used in our experiments. Furthemore, acquired knowledge would result in targeted use in livestock production, veterinary practice as well as human reproductive medicine.
1. To evaluate the effect of diet supplementation by PUFAs on reproductive parameters of female mice. 2. To evaluate the mechanisms underlying impact of PUFAs on ovulation and early embryonic development in mouse. 3. To evaluate the protective potential of diet supplementation by PUFAs in reproductive disorders.
Burkuš, J. et al. (2015) The Journal of reproduction and development. doi: 10.1262/jrd.2015-012 Čikoš, Š. et al. (2019) Biology of Reproduction, 100(2), pp. 351–364. doi: 10.1093/biolre/ioy196. Diskin, M. G. et al. (2016) Reproduction, Fertility and Development, 28(2), p. 83. doi: 10.1071/RD15366. Geisert, R. D. and Schmitt, R. A. M. (2002) Journal of Animal Science, 80(E-suppl_1), pp. E54–E65. doi: 10.2527/ANIMALSCI2002.0021881200800ES10009X. Leroy, J. L. M. R. et al. (2013) Anim. Reprod., 10, n.3, pp.258-267. https:/ Šefčíková, Z. et al. (2018) Toxicology, 410, pp. 214–221. doi: 10.1016/j.tox.2018.08.008. Šefčíková, Z. et al. (2021) Reproductive Toxicology, 105, pp. 62–71. doi: 10.1016/j.reprotox.2021.08.006. Vlčková, R. et al. (2018) Animal reprod. science, 193, pp. 171–181. doi: 10.1016/J.ANIREPROSCI.2018.04.066. Vlčková, R., Sopková, D., et al. (2022) Life, 12(8). doi: 10.3390/life12081152. Vlčková, R., Andrejčáková, Z., et al. (2022) General physiology and biophysics, 41(3), pp. 205–219. doi: 10.4149/GPB_2022003.
RNDr. Janka Babeľová, PhD.
RNDr. Veronika Kovaříková, PhD.